Figure 3From: Alterations of T cell activation signalling and cytokine production by postmenopausal estrogen levelsActivation-induced phosphorylation of CD3-zeta at physiologic estradiol concentrations. Representative western immunoblotting results demonstrating the major phosphorylated CD3-zeta bands. Jurkat cells were incubated for 48 hours in 0, 4 or 40 pg/ml estradiol and then activated by cross-linking the TcR. Cellular CD3-zeta was immunoprecipitated and the phosphorylated bands were identified using anti-pCD3-zeta as the primary antibody.Back to article page