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Figure 3 | Immunity & Ageing

Figure 3

From: Alterations of T cell activation signalling and cytokine production by postmenopausal estrogen levels

Figure 3

Activation-induced phosphorylation of CD3-zeta at physiologic estradiol concentrations. Representative western immunoblotting results demonstrating the major phosphorylated CD3-zeta bands. Jurkat cells were incubated for 48 hours in 0, 4 or 40 pg/ml estradiol and then activated by cross-linking the TcR. Cellular CD3-zeta was immunoprecipitated and the phosphorylated bands were identified using anti-pCD3-zeta as the primary antibody.

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