Detection of CXCR1, CD62L and CD11b on blood neutrophil. Peripheral blood was obtained from diseased (black and white squares) and control (black and white circles) elderly and young groups and granulocytes were purified as described in the Material and Methods section. The cells were gated on neutrophils (representative picture, 2A) via their forward (FSC) and side scatter (SSC) properties. Mean fluorescence intensity (MFI) of B, CXCR1, C, CD62L, and D, CD11b from all groups was evaluated by flow cytometry. The results are expressed as the mean of MFI ± SEM for elderly (n = 14) and young (n = 14) patients or aged (n = 14) and young (n = 14) control subjects tested individually. The data were analyzed by unpaired t-test and the value of P was considered significant when <0.05 for young control compared to patients and elderly control.