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Figure 5 | Immunity & Ageing

Figure 5

From: Ageing exacerbates damage of systemic and salivary neutrophils from patients presenting Candida-related denture stomatitis

Figure 5

Phagocytosis of viable Candida albicans by salivary and blood neutrophils from patients with Candida -wrelated denture stomatitis and controls. Saliva and blood samples from diseased (black and white squares) and control (black and white circles) elderly and young groups were collected and purified neutrophils were challenged with viable blastoconidia of C. albicans ATCC 10231 that had been opsonized with human serum (at a ratio of 1:1 of opsonized yeast: viable neutrophil). After 30 and 120 min, percentage of salivary (A) and blood neutrophils (B) phagocytosing C. albicans was assessed through staining with acridine orange and counting of 10 random fields. Results are expressed as the mean Ā± SEM from each donor analyzed individually. The data were analyzed by one-way ANOVA followed by Tukey's test and the difference was considered significant when P < 0.05. Differences are indicated by * young and elderly control groups; ** D.S. and control elderly groups; *** D.S. elderly and D.S. young groups and #D.S. and control young groups.

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