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Table 5 Blood mononuclear cell functions at basal state (T 0 ) and after supplementation with 13-cis-retinoic acid (T 1 )

From: Neutrophils are immune cells preferentially targeted by retinoic acid in elderly subjects

Parameters Incubation Young adults Elderly  
  Time T0 T 1 T0 T1 ANOVA
IL2 production 48 h 0.57 ± 0.24 0.75 ± 0.22 1.95 ± 0.75 0.77 ± 0.18 -
sIl-2R production 48 h 168 ± 24 186 ± 25 174 ± 27 201 ± 28 -
Membrane expression of CD25 (%) 48 h 27.5 ± 3.7 27.1 ± 2.8 21.3 ± 2.9 20.9 ± 2.4 -
  96 h 28 ± 2.9 34.4 ± 3.1 32.7 ± 3.9 32.4 ± 3.9 -
Number of CD25 receptors per cell 48 h 1214 ± 51 1366 ± 75 1157 ± 46 1262 ± 68 -
  96 h 874 ± 12 952 ± 22 1064 ± 67 1089 ± 27 A
  1. X ± SEM; n = 20 subjects per group. Two-way repeated-measures ANOVA was performed to discriminate among the effects of age (A; p < 0.05), nutritional treatment (N; p < 0.05) and their interaction (I; p < 0.05).
  2. Il-2 and sIl-2R productions were determined in culture media and expressed in fmol/106 cells
  3. As no significant interaction was found, comparison of marginal means was carried out, which indicated a significant effect of age on number of CD25 receptors per cell (96 h) (young: 914.03 ± 14.16 vs elderly: 1077.24 ± 34.21)