Neutrophils are immune cells preferentially targeted by retinoic acid in elderly subjects

Immunity & Ageing

Table 5 Blood mononuclear cell functions at basal state (T₀) and after supplementation with 13-cis-retinoic acid (T₁)

Parameters	Incubation	Young adults		Elderly
	Time	T₀	T ₁	T₀	T₁	ANOVA
IL2 production	48 h	0.57 ± 0.24	0.75 ± 0.22	1.95 ± 0.75	0.77 ± 0.18	-
sIl-2R production	48 h	168 ± 24	186 ± 25	174 ± 27	201 ± 28	-
Membrane expression of CD25 (%)	48 h	27.5 ± 3.7	27.1 ± 2.8	21.3 ± 2.9	20.9 ± 2.4	-
	96 h	28 ± 2.9	34.4 ± 3.1	32.7 ± 3.9	32.4 ± 3.9	-
Number of CD25 receptors per cell	48 h	1214 ± 51	1366 ± 75	1157 ± 46	1262 ± 68	-
	96 h	874 ± 12	952 ± 22	1064 ± 67	1089 ± 27	A

X ± SEM; n = 20 subjects per group. Two-way repeated-measures ANOVA was performed to discriminate among the effects of age (A; p < 0.05), nutritional treatment (N; p < 0.05) and their interaction (I; p < 0.05).
Il-2 and sIl-2R productions were determined in culture media and expressed in fmol/10⁶ cells
As no significant interaction was found, comparison of marginal means was carried out, which indicated a significant effect of age on number of CD25 receptors per cell (96 h) (young: 914.03 ± 14.16 vs elderly: 1077.24 ± 34.21)

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