Age dependent cytokine production from mouse BMM in response to Pg. Cytokine analysis in cell culture supernatant fluids of mouse BMM from 2-months (n = 6), 1-year (n = 3) and 2-years (n = 3) of age. Cells were cultured with Pg 381 (filled bars), Pg DPG3 (gray bars) at multiplicity of infection (MOI) of 100, or medium only control (open bars) for 24 h. Cytokine levels were determined by multiplex analysis and the data presented as pg/mL mean ± standard error of the mean. A. TNF-α; B. IL-1β; C. IL-6; and D. IL-10. Asterisks indicate statistically significant (P < 0.05) as determined by Two way ANOVA with Bonferroni post-test. NS = not significant.