Chemokine response of young and aged mouse BMM to Pg. Chemokine levels in cell culture supernatant fluids of mouse BMM from 2-months (n = 6), 1-year (n = 3) and 2-years (n = 3) of age. BMM were challenged with Pg 381 (filled bars), Pg DPG3 (gray bars) at multiplicity of infection (MOI) of 100, or medium only control (open bars) for 24 h. Chemokine levels were determined by multiplex analysis and the data presented as pg/mL mean ± standard error of the mean. A. KC; B. MCP-1; C. MIP-1α; and D. RANTES. Asterisks indicate statistically significant (P < 0.05) as determined by Two way ANOVA with Bonferroni post-test analysis. NS = not significant.