Prostaglandin E2 levels from young and aged mouse BMM in response to Pg. PGE2 present in cell culture supernatant fluids of mouse BMM from 2-months (n = 6), 1-year (n = 3) and 2-years (n = 3) of age. Cells were challenged with Pg 381 (filled bars), Pg DPG3 (gray bars) at multiplicity of infection (MOI) of 100, or medium only control (open bars) for 24 h. PGE2 levels were measured by ELISA and the data presented as pg/mL mean ± standard error of the mean. Statistically was determined by Two way ANOVA with Bonferroni post-test analysis. NS = not significant.