Figure 2

Co-localization of IGF-1R and Fyn within synaptic zone during traumatic stress. 2-month and 1-year mice were killed 1 and 3 days after traumatic stress (n = 5 for each group), cross section of frontal cortex were immunostained using anti-IGF-1R and anti-Fyn antibodies, and double-labeled cells were identified using a Leica Q500IW image analysis system (A). The graph depicts expressions as percentages of controls (B). Pre-synaptic (C) and PSD regions (D) were isolated, and association of IGF-1R with Fyn was determined by immunoprecipitation assay. The immunoprecipitation antibody was anti-Fyn and the immunoblotting antibody was anti-IGF-1R. Data were normalized and calculated as percentage of control, values represent mean±SD for 3 independent experiments. Con: control; T1 and 3: 1 and 3 days after trauma. *p<0.05 vs Con. Scale bars, 50μm.