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Figure 3 | Immunity & Ageing

Figure 3

From: Age-Dependent Neuroimmune Modulation of IGF-1R in the Traumatic Mice

Figure 3

Subcellular distribution of IGF-1R during traumatic stress. Fyn (+/−) and Fyn (−/−) mice were killed 1 and 3 days after traumatic stress (n = 5 for each group), a lipid raft from frontal cortex was prepared to determine subcellular distribution of IGF-1R. Western blot analysis was used to detect IGF-1R expression in fractions 2–10, and CTB immunopositive fractions were identified as lipid raft fractions (A). Data were calculated as percentage of total, each value represents mean± SD for 3 independent experiments (B). Fyn (+/−) and Fyn (−/−) mice were killed 1 and 3 days after traumatic stress (n = 5 for each group), a lipid raft from frontal cortex was prepared, and association of IGF-1R with CTB was determined by immunoprecipitation assay. The immunoprecipitation antibody was anti-CTB and the immunoblotting antibody was anti-IGF-1R. Data were normalized and calculated as percentage of control, values represent mean±SD for 3 independent experiments (C). 2-month and 1-year Fyn (+/−) were killed 1 and 3 days after traumatic stress (n = 5 for each group), a lipid raft from frontal cortex was prepared and immunoprecipitated using anti-Fyn (1:200), 32P incorporation in the resulting pellets were determined by incubating with 5 μg of Src substrate peptide in kinase buffer at 30°C, data was converted to pmol/min, values represent mean±SD for 3 independent experiments (D). Con: control; T1 and 3: 1 and 3 days after trauma. *p<0.05 vs Con.

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