Figure 4

IGF-1R/Fyn signaling within neuronal lipid rafts. Cortical neurons from Fyn (+/−) and Fyn (−/−) mice were cultured for 10 days. A lipid raft was prepared to determine sucellular distribution of IGF-1R. Western Blot analysis was used to detect IGF-1R expression in fractions 2–9, and CTB immunopositive fractions were identified as lipid raft fractions (A). Data were calculated as percentage of total, each value represents mean± SD for 3 independent experiments (B). Cortical neurons from Fyn (+/−) mice were cultured for 10 and 50 days. Neurons were treated with vehicle or IL-1β, IL-1ra for the indicated time, and then assessed for the presence of IGF-1R in CTB material by immunoprecipitation. Data were normalized and calculated as percentage of control, each value represents mean± SD for 5 independent experiments (C). Fyn (+/−) and Fyn (−/−) mice were killed Wild type and Fyn (−/−) mice were 1 and 3 days after traumatic stress (n = 5 for each group), synaptoneurosome in the frontal cortex were extracted, IGF-1R expression was measured by Western Blot analysis. Data were normalized and calculated as percentage of control, each value represents mean±SD for 3 independent experiments (D). *p<0.05 vs Con. Con: control; T1 and 3: 1 and 3 days after trauma.