Figure 5

Modulation of IGF-1R/Fyn signaling within the synaptic zone during traumatic stress. 2-month and 1-year mice were subjected to surgical trauma, some of these mice were injected icv with IGF-1. Thus, 5 groups of mice were created: Con, T1, T1 + IGF-1, T3, T3 + IGF-1 (n = 5 for each group). Pre-synaptic and PSD fraction from frontal cortex were prepared and assessed for IGF-1R expression using Western Blot (A and B). The interaction of IGF-1R and Fyn was assessed by immunoprecipitation (C and E). Panel D and F depict quantitative analysis of C and E respectively. Data were calculated as percentage of control, each value represents mean±SD for 3 independent experiments (D). *p<0.05 vs Con. Con: control; T1 and 3: 1 and 3 days after trauma.