Figure 2

Zizimin2, Zizimin1, and Zizimin3 expression in young and aged mice. A) Total RNA was prepared from young (7-8 weeks old) or aged (24 months old) mice, followed by reverse transcription to obtain the cDNA. Quantitative RT-PCR was carried out with specific primer sets for Zizimin2 and G3PDH. Relative mRNA levels were calculated using the delta-delta CT method using G3PDH as a housekeeping gene. Asterisks indicate that the sample group is significantly different from aged mice at *** P < 0.0001 by Student's t-test. Means ± S.D. values of four experiments are presented. B) Lysates were prepared from three individual three young (7-8 weeks old) or aged (24 months old) mouse tissues, which are described to the left of each panel, followed by western blotting. α-Tubulin was used as a loading control. C) Expression levels of Zizimin2 and Zizimin1 were quantified as relative levels. Means ± S.D. values of three experiments are presented. ** P < 0.01 by Student's t-test.