Skip to main content
Figure 2 | Immunity & Ageing

Figure 2

From: Defective pro-IL-1β responses in macrophages from aged mice

Figure 2

Efficient processing of pro-IL-1β in BMM from aged mice. BMM were primed with LPS (200 ng/ml) for 3 hours or primed and stimulated with ATP (5 mM) for one hour (LPS + ATP). Intracellular production of pro-IL-1β as measured by GMFI was assessed in control, LPS and LPS + ATP treated BMM from young and aged mice using the NJTEN3 Ab at 0.05 μg per 106 cells (eBiosciences) (A/B). Controls were incubated with cell culture medium. ELISA of extracellular active IL-1β in supernatants of control, LPS and LPS + ATP treated BMM from young and aged mice (C). Data shown are mean values from triplicate wells +/− SEM. Representative data of at least 3 experiments each performed in triplicate. (* = P <0.05, ** = P < 0.01 Mann-Whitney Test).

Back to article page