Figure 3

Age related differences in IL-1β secretion correlate with intracellular pro-IL-1β content. Time course of intracellular pro-IL-1β production levels in LPS (200 ng/ml) stimulated BMM from young and aged mice using flow cytometry (A). ELISA of IL-1β (B and C) and IL-18 (D and E) levels in supernatants of young and aged BMM stimulated with LPS (200 ng/ml) for 3 and 24 hours respectively and ATP (5 mM) for 1 hour. Controls were incubated with cell culture medium. Data shown are the mean values from triplicate wells +/− SEM. Representative data of at least 3 experiments each performed in triplicate.