Figure 4

Mice with 5HNP _324-332 /K^bperturbations exhibit memory T cell populations that are less clonally diverse. Representative flow cytometric plots from Sendai virus infected mice (<1yr post infection) with a small perturbation (A and B) or normal memory T cell repertoire (C and D). Frequency of NP_324-332K^b+ (A and C, left plot), 5HNP_324-332K^b+ (A and C, right plot) and their Vβ8 usage (B and D) are shown. Plots are gated on CD8⁺ (A and C) or tetramer⁺ (B and D) populations. Numbers in the gate indicate frequency of cells among CD8⁺ T cells, numbers outside the gate represent frequency of cells relative to frequency of NP_324-332K^b+ cells.