SENIEUR status of the originating cell donor negates certain ‘anti-immunosenescence’ effects of ebselen and N-acetyl cysteine in human T cell clone cultures

Table 1 GSH:GSSG ratio of TCCs + / - Ebselen

Times in culture (Weeks)	30μM ebselen	400-23 (26 yr ol)	385-7 (45 yr old)	399-37 (80 yr old)
2	-	42.3 ± 1.7	44.3 ± 2.4	37.7 ± 0.4*
2	+	44.0 ± 1.7	41.9 ± 1.2	36.7 ± 0.9*
5	-	44.2 ± 2.8	42.0 ± 1.8	30.8 ± 2.0*
5	+	47.3 ± 2.4	46.8 ± 5.1	31.3 ± 1.5*
9/10	-	36.1 ± 1.6	33.4 ± 4.1	25.5 ± 0.7*
9/10	+	37.2 ± 1.4	36.3 ± 2.9	26.6 ± 0.8*

GSH:GSSG ratios in three pooled CD4⁺ TCCs (+ / - 30 μM Ebselen) derived from a healthy 80 year old donor are significantly lower than in either of the three pooled TCCs, each derived from a healthy 26 or a 45 year old donor (published data from [10]).
*Significantly lower GSH:GSSG ratio in 399-37 clones (80 year old) compared to either 400-23 (26 year old) or 385-7 (45 year old) clones.

ISSN: 1742-4933