SENIEUR status of the originating cell donor negates certain ‘anti-immunosenescence’ effects of ebselen and N-acetyl cysteine in human T cell clone cultures

Tables 4 Levels of oxidative DNA damage in TCCs on antioxidant supplementation

Time in culture (Weeks)	30 μM Ebselen (% of DNA in comet tail)
	-			+
	Alk	End	Fpg	Alk	End	Fpg
2	19.7 ± 0.6	21.6 ± 0.6	22.5 ± 0.6	20.6 ± 0.8	22.5 ± 0.8	23.5 ± 0.8
5	25.6 ± 0.6	27.6 ± 0.4	31.7 ± 0.8	26.3 ± 1.2	30.1 ± 1.0	32.0 ± 1.1
10	46.9 ± 0.6	48.8 ± 0.7	51.2 ± 1.2	48.8 ± 0.7	49.8 ± 0.6	51.8 ± 2.1
	*	*	*	*	*	*
Time in culture (Weeks)	7.5 mM NAC (% of DNA in comet tail)
	-			+
	Alk	End	Fpg	Alk	End	Fpg
2	21.8 ± 0.8	22.6 ± 0.6	23.7 ± 0.3	22.2 ± 0.3	23.9 ± 0.1	24.2 ± 0.2
5	28.2 ± 1.6	30.8 ± 0.8	33.0 ± 1.2	27.7 ± 1.2	32.9 ± 0.8	34.1 ± 0.3
10	48.7 ± 0.4	49.9 ± 0.7	52.6 ± 1.0	50.2 ± 0.3	52.2 ± 1.2	53.6 ± 0.9
10	*	*	*	*	*	*

The increase in levels of oxidative DNA damage in CD4⁺ TCC derived from a healthy 80 year old donor supplemented + / - 30 μM Ebselen or 7.5 mM NAC.
*Significantly higher levels of oxidative DNA damage in week 10 on comparison with the earlier weeks (2 and 5). n = 3 pooled TCCs for each age group.

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