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Tables 4 Levels of oxidative DNA damage in TCCs on antioxidant supplementation

From: SENIEUR status of the originating cell donor negates certain ‘anti-immunosenescence’ effects of ebselen and N-acetyl cysteine in human T cell clone cultures

Time in culture (Weeks) 30 μM Ebselen (% of DNA in comet tail)
- +
Alk End Fpg Alk End Fpg
2 19.7 ± 0.6 21.6 ± 0.6 22.5 ± 0.6 20.6 ± 0.8 22.5 ± 0.8 23.5 ± 0.8
5 25.6 ± 0.6 27.6 ± 0.4 31.7 ± 0.8 26.3 ± 1.2 30.1 ± 1.0 32.0 ± 1.1
10 46.9 ± 0.6 48.8 ± 0.7 51.2 ± 1.2 48.8 ± 0.7 49.8 ± 0.6 51.8 ± 2.1
  * * * * * *
Time in culture (Weeks) 7.5 mM NAC (% of DNA in comet tail)
- +
Alk End Fpg Alk End Fpg
2 21.8 ± 0.8 22.6 ± 0.6 23.7 ± 0.3 22.2 ± 0.3 23.9 ± 0.1 24.2 ± 0.2
5 28.2 ± 1.6 30.8 ± 0.8 33.0 ± 1.2 27.7 ± 1.2 32.9 ± 0.8 34.1 ± 0.3
10 48.7 ± 0.4 49.9 ± 0.7 52.6 ± 1.0 50.2 ± 0.3 52.2 ± 1.2 53.6 ± 0.9
* * * * * *
  1. The increase in levels of oxidative DNA damage in CD4+ TCC derived from a healthy 80 year old donor supplemented + / - 30 μM Ebselen or 7.5 mM NAC.
  2. *Significantly higher levels of oxidative DNA damage in week 10 on comparison with the earlier weeks (2 and 5). n = 3 pooled TCCs for each age group.