Skip to main content
Figure 1 | Immunity & Ageing

Figure 1

From: Blood–brain barrier dysfunction developed during normal aging is associated with inflammation and loss of tight junctions but not with leukocyte recruitment

Figure 1

Blood–brain barrier integrity and neuroinflammation. (A) The integrity of BBB was assessed by measuring the blood-to-brain extravasation of IgG with semi-quantitative confocal microscopy in the cortex (CTX) and hippocampal formation (HPF) of 3 months old young mice and 24 months old aged mice. The voxel intensity of protein of interest is expressed as per volume unit. Asterisks indicate statistical significance assessed with two-tailed t-test (p < 0.05, n = 6). Representative immunomicrographs are also shown (green = IgG, blue = DAPI, scale bar = 20 μm). (B) The astroglial activation, neuronal ER stress and inflammation were assessed by measuring the expression of GFAP, GRP78 and COX-2 respectively. Representative immunomicrographs are shown (yellow = GFAP, red = GRP78, magenta = COX-2, blue = DAPI, scale bar = 50 μm).

Back to article page

Immunity & Ageing

ISSN: 1742-4933

Contact us