Fig. 2

CASAC enhances CD8+ T cell responses to a foreign antigenic epitope in aged mice. Young (open symbols) and aged (filled symbols) C57BL/6 mice were vaccinated twice with the SIL peptide from OVA in combination with either CFA/IFA (circles) or CASAC (triangles). a The percentage of H-2K^b/SIL-pentamer + CD8+ T cells was assessed after staining of peripheral blood samples with H-2K^b/SIL-pentamer. b Absolute numbers of H-2K^b/SIL-pentamer + CD8+ T cells were enumerated by flow cytometry. c % Lysis of target cells was assessed using CFSE^low–SIL-loaded and CFSE^high-SVL-loaded splenocytes, as antigen-specific and control targets, respectively. Both populations were mixed and injected iv into the immunised and control mice. Eighteen hours later, all spleens were harvested and splenocytes were analysed for their CFSE content by flow cytometry. The percent target lysis was calculated with the following formula: % lysis = 1 – [(number of CFSE^low/ number of CSFE^high in immunised animal)/(number of CFSE^low/ number of CSFE^high in unimmunised animal)] × 100. Each symbol represents an individual mouse and the median is indicated by a horizontal line. Data is pooled from 2 independent experiments. The Mann-Whitney U test was used to compare distributions