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Table 1 HIV patients stable on ART retain elevated humoral and cellular responses to CMV and advanced T-cell senescence

From: The immunological footprint of CMV in HIV-1 patients stable on long-term ART

  HIV patients CMV + Controls CMV- Controls A vs B A vs C B vs C
A B C P b P b P b
n 20 16 9    
Male:Female 19:1 14:2 9:0    
Age (years) 62.5 (50–73)a 60 (50–74)a 55(52–69)a 0.19 0.03 0.12
Levels in Plasma       
CMV lysate antibody AU/L 94 (23–995) 20 (6–83) 0.8 (0.5–1.1) <0.0001 <0.0001 <0.0001
CMV gB antibody AU/L 127 (27–400) 45 (2–88) 2 (0.6–3.3) <0.0001 <0.0001 <0.0001
CMV IE-1 antibody AU/L 49 (8–1098) 9 (2–180) 2.6 (1.9–10) <0.0001 <0.0001 0.003
sCD14 ng/mL 22 (7.7–48) 18 (8.3–39) 22 (11–31) 0.09 0.23 0.68
sTNFR1 pg/mL 15 (9.1–25) 15 (12–27) 17 (10–21) 0.68 0.83 0.72
Total IgG mg/mL 12 (3–22) 12 (5–20) 9.4 (6.5–15) 0.46 0.16 0.14
sBAFF ng/mL 740 (376–1401) 519 (274–786) 319 (318–471) 0.002 0.0003 0.06
IFNγ spots per 2 × 106 cells       
CMV lysate 227 (16–700) 157 (13–617) 0 (0–0.5) 0.16 <0.0001 <0.0001
CMV pp65 445 (14–1591) 138 (18–645) 0 (0–2) 0.005 <0.0001 <0.0001
CEF control peptide pool 516 (2–1500) 337 (20–584) 4 (0–404) 0.07 0.001 0.0015
NLV peptide 498 (56–1363)c 214 (13–651)d na 0.06 na na
VLE peptide 420 (14–2000)c 25 (7–561)d na 0.005 na na
T cell subset [as % of]       
CD4+ T cells [lymphocytes] 43 (24–77) 69 (52–84) 69 (52–80) <0.0001 0.0009 0.93
CD57+ [CD4] 11 (2–75) 8 (2–26) 4.5 (1.7–7.4) 0.11 0.001 0.03
CD57+CD45RA+CD27 [CD4] 1.9 (0–57) 0.44 (0.06–14) 0.02 (0.005–0.16) 0.06 0.0002 0.0001
CD8+ T cells [lymphocytes] 48 (16–71) 22 (7–43) 21 (15–44) 0.0001 0.002 0.97
CD57+ [CD8] 47 (17–67) 40 (6.4–69) 28 (10–68) 0.08 0.04 0.51
CD57+CD45RA+CD27 [CD8] 19 (4.2–53) 26 (4–49) 8 (2–19) 0.61 0.03 0.0001
  1. na = Not applicable as none of the CMV-seronegative healthy controls carried the HLA-A*02 allele
  2. aMedian (range)
  3. bMann–Whitney, P ≤ 0.05 (bold), P > 0.05-0.1 (italics)
  4. cHLA-A*02 allele restricted thus HIV+ patients n = 11
  5. dCMV+ controls n = 9