Fig. 6

Aging increases the number of all spinal cord mononuclear cells, and CD45^int/hi CD11b + nonlymphoid cells, recovered on the 7^th d.p.i. from AO rat spinal cord. (a) Bar graph indicates the total number of mononuclear cells (MNC) isolated from spinal cord of young and aged rats on the 7^th d.p.i. (Panel b) Flow cytometry density plots indicate the expression of CD45 on CD11b + cells from spinal cord of (left) young and (right) aged rats on the 7^th d.p.i. CD11b + cells were gated as shown in the flow cytometry histograms. Numbers in the flow cytometry histograms represent the percentage of CD11b + cells. R1 = CD11b + CD45^hi cells; R2 = CD11b + CD45^int cells. Numbers in the table indicate the percentage of cells in the specified region (R). Bar graph indicates the number of CD11b + cells in spinal cords from young and aged rats. (Panel c) Bar graphs indicate the number of (left) CD11b + CD45^int and (right) CD11b + CD45^hi cells in spinal cords from young and aged rats on the 7^th d.p.i. (d) Bar graph indicates the fold change in expression of mRNA for SOCS1 in spinal cord mononuclear cells from aged relative to young rats on the 7^th d.p.i. as determined by RT-qPCR. Data are normalized to β-actin (ACTB). All results are presented as means ± SEM (n = 9/group). Data are representative of one of two experiments with similar results. *p < 0.05; ***p < 0.001