Skip to main content

Advertisement

Fig. 6 | Immunity & Ageing

Fig. 6

From: Aging diminishes the resistance of AO rats to EAE: putative role of enhanced generation of GM-CSF Expressing CD4+ T cells in aged rats

Fig. 6

Aging increases the number of all spinal cord mononuclear cells, and CD45int/hi CD11b + nonlymphoid cells, recovered on the 7th d.p.i. from AO rat spinal cord. (a) Bar graph indicates the total number of mononuclear cells (MNC) isolated from spinal cord of young and aged rats on the 7th d.p.i. (Panel b) Flow cytometry density plots indicate the expression of CD45 on CD11b + cells from spinal cord of (left) young and (right) aged rats on the 7th d.p.i. CD11b + cells were gated as shown in the flow cytometry histograms. Numbers in the flow cytometry histograms represent the percentage of CD11b + cells. R1 = CD11b + CD45hi cells; R2 = CD11b + CD45int cells. Numbers in the table indicate the percentage of cells in the specified region (R). Bar graph indicates the number of CD11b + cells in spinal cords from young and aged rats. (Panel c) Bar graphs indicate the number of (left) CD11b + CD45int and (right) CD11b + CD45hi cells in spinal cords from young and aged rats on the 7th d.p.i. (d) Bar graph indicates the fold change in expression of mRNA for SOCS1 in spinal cord mononuclear cells from aged relative to young rats on the 7th d.p.i. as determined by RT-qPCR. Data are normalized to β-actin (ACTB). All results are presented as means ± SEM (n = 9/group). Data are representative of one of two experiments with similar results. *p < 0.05; ***p < 0.001

Back to article page