Fig. 1

Impaired motor and sensory functions in adult obese, aged and aged obese mice. a) Body weight changes were observed in DIO mice, significantly increased in both DIO 6 and 17 month-mice compared to age-matched lean mice. (n = 6–8/group, ***p < 0.001, lean 6 vs. DIO 6, +++p < 0.001, lean 17 vs. DIO 17, ###p < 0.001, DIO 6 vs. DIO 17, ^xxx p < 0.001, lean 3 vs. lean 6, 17). b) Blood glucose levels were not significantly altered after long term high-fat diet (n = 6–8/group). c) Impaired motor coordination was observed in both DIO 6 and 17 month-mice, while lean 17 month-mice stayed less time on Rotarod. (n = 6–8/group, ***p < 0.001, lean 6 vs. DIO 6, +++p < 0.001, lean 17 vs. DIO 17,) d) Mechanical allodynia was reversed with aging (^xxx p < 0.001, lean 3 vs. lean17) and with obesity in 6 month-mice (n = 6–8/group, *p < 0.05, lean 6 vs. DIO 6). e) Thermal hypersensitivity was observed in DIO 6 month-mice compared to lean 6 month-mice (n = 6–8/group, **p < 0.01, lean 6 vs. DIO 6) as well as in aged mice (n = 6–8/group, ^xx p < 0.01, ^xxx p < 0.001, lean 3 months vs. lean 6, 17 months). f) Cold hypersensitivity was detected in DIO 6 month-mice (n = 6–8/group, **p < 0.01, lean 6 vs. DIO 6), however, it was significantly reversed in DIO 17 month-mice (n = 6–8/group, +++p < 0.001, lean 17 vs. DIO 17, ###p < 0.001, DIO 6 vs. DIO 17)