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Table 1 Detailed information on the selection of primers for real-time reverse-transcriptase polymerase chain reaction

From: Characteristics of spinal microglia in aged and obese mice: potential contributions to impaired sensory behavior

Gene NCBI ID # Forward Reverse
Primer Sequence Primer Sequence
IL1β NM_008361.3 mIL1β-F1 CTATACCTGTCCTGTCTA mIL1β-R1 GCTCTTGACTTCTATCTTG
IL6 NM_031168.1 mIL6-F1 CTGAAACTTCCAGAGATA mIL6-R1 TTCATGTACTCCAGGTAG
TNFα NM_013693.2 mTNFα-F1 TTCTGTCTACTGAACTTC mTNFα-R1 CCATAGAACTGATGAGAG
IL10 NM_010548.2 mIL10-F1 CTATGCTGCCTGCTCTTA mIL10-R1 GCTGGTCCTTTGTTTGAAA
TGFβ NM_009367.3 mTGFβ-F1 AGAGAAGAACTGCTGTGT mTGFβ-R1 GGTTGTGTTGGTTGTAGAG
GAPDH NM_008084.2 mGAPDH-F1 GTGAAGGTCGGTGTGAAC mGAPDH-R1 AATCTCCACTTTGCCACTG
  1. NCBI National Center for Biotechnology Information
  2. IL interleukin
  3. TNF tumor necrosis factor
  4. TGF transforming growth factor
  5. GAPDH glyceraldehyde 3-phosphate dehydrogenase