Fetal thymus graft enables recovery from age-related hearing loss and expansion of CD4-Positive T cells expressing IL-1 receptor type 2 and regulatory T Cells

Table 1 Evaluation of the major T cell population

Groups	CD4⁺CD3⁺ (%)	CD8⁺CD3⁺ (%)
C (untreated mice of 12 months old)	10.4 ± 0.08	3.85 ± 0.21
D (thymus-grafted mice of 12 months old)	12.9 ± 0.26^*	3.86 ± 0.36^a

To evaluate the major T cell population, CD4⁺CD3⁺ T cells and CD8⁺CD3⁺ T cells were analyzed by flow cytometry after the staining with FITC-anti-mouse CD4 mAb plus PE-anti-mouse CD3 mAb, or FITC-anti-mouse CD8 mAb plus PE-anti-mouse CD3 mAb
^*The frequency of CD4⁺CD3⁺T cells in Group D was significantly higher than those in Group C (p < 0.002)
^aThere was no significant difference of the frequencies of CD8⁺ CD3⁺T cells between Groups C and D. All experiments were in triplicate n = 3/group (mean ± SD)

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