Fig. 1
From: End stage renal disease patients have a skewed T cell receptor Vβ repertoire
Skewed and non-skewed TCR Vβ repertoire distribution in healthy individuals (HI) and end stage renal disease (ESRD) patients. The different genescan profiles obtained following spectratyping of the DNA-based multiplexed TCR Vβ PCR samples are depicted in a. This reaction consists of 23 Vβ primers and 9 Jβ primers. The green profile represents the 6 Jβ primers (Jβ1.1, Jβ1.2, Jβ1.3, Jβ1.4, Jβ1.5, Jβ1.6), whereas the blue line represents the 3 Jβ primers (Jβ2.2, Jβ2.6, and Jβ2.7) in this multiplex PCR. The red profile represents the internal size standard. The left profile shows a non-skewed (polyclonal) pattern, the middle one shows a skewed pattern with a single (oligo)clonal peak (size of the oligoclonal product is 253 nucleotides (nt)) whereas the right panel depicts a skewed pattern with multiple (oligo)clonal peaks (sizes of the oligoclonal products are 252, 261 and 264 nt, respectively). The arrows indicate oligoclonal peaks within the genescan profile. b Frequency of ESRD patients (n = 45) and age-matched HI (n = 51) with a skewed (closed squares) and non-skewed (open squares) TCR Vβ repertoire. c Frequency of single (open squares) and multiple (oligo)clonal peaks (closed squares) within a skewed TCR Vβ repertoire of ESRD patients (n = 31) and HI (n = 16). P value: * <0.05; ** < 0.01; *** < 0.001; NS: not significant