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Fig. 1 | Immunity & Ageing

Fig. 1

From: Antibodies to the DNA-directed RNA polymerase II subunit RPB1 occur with highest frequency in centenarians

Fig. 1

Defining the humoral repertoire of centenarians with peptide mimotopes. a Microtiter plates were coated with anti-human IgG antibodies. After blocking, sera from individual centenarians (Centenarian group), healthy volunteers aged 60–79 years (Old group), and healthy volunteers younger than or equal to 43 years (Young group) were added to the wells. Phage-displaying peptide YSATLRY or YSPTLFY was added, and the amount of bound phage was determined using the anti-M13 antibody HRP conjugate and ABTS substrate solution. (b) Microtiter plates were coated with BSA-conjugated peptides (YSA = YSATLRYGGGSC, YSP = YSPTLFYGGGSC). After blocking, sera from individuals were added to each well. After incubation and washing, HRP-conjugated anti-human IgG (H + L) antibodies and ABTS solution were added sequentially with intermittent washing. * P < 0.05. (c) R-square calculation from the enzyme immunoassay result shown in B (R 2 = 0.94)

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