Fig. 5

Phagocytosis decreases in chico mutant flies. a Representative images of phagocytosis in Drosophila melanogaster chico mutants and yellow white (yw) background control 7-10 day old adult flies at 60 min after injection of pHrodo-labeled Escherichia coli particles. Images were taken using fluorescence microscopy at 10x magnification. b Corrected total fluorescence in chico mutant and control flies (n = 7) at 60 min following injection of pHrodo-labeled E. coli particles. Images were processed in ImageJ and corrected total fluorescence was estimated by measuring relative amounts of fluorescence, which involved estimations of the resulting area, mean fluorescence of background and integrated density. Phagocytosis experiments were repeated three times. Values shown are means and error bars represent standard deviations **P < 0.01 (unpaired two-tailed t-test, GraphPad Prism5 software)