Fig. 4

a Regional distribution of HO-1 in rat brain. HO-1 protein levels in different brain regions of rats fed H. erinaceus biomass preparation as compared to control group. Brain region homogenates from control and mushroom supplemented rats were assayed for HO-1 expression by Western blot. The bar graph shows densitometric values, expressed as mean standard error of mean of 3 independent analyses. P < 0.05 vs control. In the representative immunoblot shown, β-actin has been used as loading control. D.U., densitometric units. CX: cortex; Hp: Hippocampus; Cb: cerebellum. b Regional distribution of Hsp70 in rat brain. Inducible Hsp70 protein levels in different brain regions of rats fed H. erinaceus biomass preparation as compared to control group. Brain region homogenates from control and mushroom supplemented rats were assayed for Hsp70 expression by Western blot. The bar graph shows densitometric values, expressed as mean standard error of mean of 3 independent analyses. P < 0.05 vs control. In the representative immunoblot shown, β-actin has been used as loading control. D.U., densitometric units. CX: cortex; Hp: Hippocampus; Cb: cerebellum. c Regional distribution of thioredoxin in rat brain. Trx protein levels in different brain regions of rats fed H. erinaceus biomass preparation as compared to control group. Brain region homogenates from control and mushroom supplemented rats were assayed for Trx expression by Western blot. The bar graph shows densitometric values, expressed as mean standard error of mean of 3 independent analyses. P < 0.05 vs control. In the representative immunoblot shown, β-actin has been used as loading control. D.U., densitometric units. CX: cortex; Hp: Hippocampus; Cb: cerebellum