Fig. 3

Effect of the inhibition of Sirt1 and Cat activities on endothelial cell senescence. SA-β-gal staining of ECs conditioned with sera from HF patients in the presence and absence of oxidative stress. Sirt1 and Cat activities were inhibited by EX-527 and 3-amino-1,2,3-triazole (ATZ), respectively. Senescence values are shown as a percentage of the reference condition (FBS-conditioned ECs), which is 100%. a) P < 0.05 vs baseline; b) P = 0.001 vs baseline; c) P < 0.05 vs H₂O₂; d) P = 0.01 vs EX-527; e) P = 0.001 vs H₂O₂; f) P < 0.02 vs EX-527; g) P < 0.0001 vs baseline; h) P < 0.01 vs EX-527;i) P < 0.05 vs EX-527; j) P < 0.05 vs ATZ; k) P < 0.02 vs EX-527 + H₂O₂; l) P < 0.0001 vs H₂O₂. (B): a) P = 0.001 vs baseline; b) P < 0.02 vs baseline; c) P < 0.005 vs baseline; d) P = 0.002 vs H₂O₂; e) P = 0.01 vs EX-527; f) P < 0.01 vs baseline; g) P < 0.05 vs EX-527; h) P < 0.05 vs H₂O₂; i) P = 0.002 vs EX-527 + H₂O₂; j) P = 0.001 vs EX-527 + H₂O₂; k) P < 0.005 vs H₂O₂