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Table 2 Confirmation of microarray results by qPCR

From: Transcriptomic evidence of a para-inflammatory state in the middle aged lumbar spinal cord

 Results

Lgals

Fcgr2b

Gpnmb

C3

Atf3

Ptprc

Cd163

Nrg1

Cd11b

ΔΔCt M v Y

1.62

1.68

1.76

1.42

0.94

0.99

0.78

−0.79

0.87

qPCR Fold M/Y

3.08

3.20

3.40

2.68

1.92

1.99

1.72

0.58

1.83

Microarray M/Y

2.44

2.89

3.14

2.40

1.73

1.84

1.59

0.46

N/A

Adjusted p-value

0.004

0.003

0.006

0.010

0.004

0.001

0.001

0.056

1.0E-07

  1. cDNA was synthesized from the same RNA samples analyzed in the microarrays. ΔΔCt values are expressed as described in Methods