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Table 2 Confirmation of microarray results by qPCR

From: Transcriptomic evidence of a para-inflammatory state in the middle aged lumbar spinal cord

 Results Lgals Fcgr2b Gpnmb C3 Atf3 Ptprc Cd163 Nrg1 Cd11b
ΔΔCt M v Y 1.62 1.68 1.76 1.42 0.94 0.99 0.78 −0.79 0.87
qPCR Fold M/Y 3.08 3.20 3.40 2.68 1.92 1.99 1.72 0.58 1.83
Microarray M/Y 2.44 2.89 3.14 2.40 1.73 1.84 1.59 0.46 N/A
Adjusted p-value 0.004 0.003 0.006 0.010 0.004 0.001 0.001 0.056 1.0E-07
  1. cDNA was synthesized from the same RNA samples analyzed in the microarrays. ΔΔCt values are expressed as described in Methods