Fig. 1

Overview of experimental design and results. a Naïve (CD4+/CD44low/int) and memory (CD4+/CD44 high) CD4 T cells were isolated from 4 young and 5 aged mice by FACS, and global gene expression levels were measured using microarray. b The number of up- and down-regulated genes differentially expressed with an FDR ≤0.05, in both naïve and memory subsets. The number in middle represents number of overlapping genes between both subsets, and is a fraction of the total number reported for both naïve and memory groups (i.e., 121 genes up-regulated in naïve cells at FDR ≤0.05, 41 of which were also upregulated in memory cells). c The number of up- and down-regulated genes differentially expressed with an expanded FDR of ≤0.1, in both naïve and memory subsets. Analysis results for the expanded gene list are referred to in the results and complete data is available in the supplemental data