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Fig. 2 | Immunity & Ageing

Fig. 2

From: Ileal antimicrobial peptide expression is dysregulated in old age

Fig. 2

a Inverse Simpson index (15.41 and 9.92 in young and old mice, respectively, Kruskal-Wallis test, p = 0.24). b Chao1 index (727.85 and 553.44 in young and old mice, respectively, Kruskal-Wallis test, p = 0.021). c Linear Discriminant scores based on LefSe analysis showing the OTU more represented in young (green) and old (red) mice. Total genomic DNA extraction and sequencing of the V4 region of the bacterial 16 s rRNA gene was performed by Microbiome Insights (Vancouver, Canada). Sequences were analyzed using the software package Mothur (version 1.38.1) [24] according to the Standard Operating Procedure [25]. Alignment was performed using Silva database v123, reduced to the V4 region. Chimeras were removed using UCHIME [26]. Sequencing errors leading to rare variants were reduced by pre-clustering sequences into groups. Non-bacterial sequences were removed and the bacterial ones were classified using the RDB trainset no. 14. Differences in the abundance of OTUs were detected using Metastats [27]

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