Fig. 5

TNF-α-induced NF-κB activity in T_N and T_CM CD8+ T cells by ELISA-based DNA binding assay. Purified T_N and T_CM CD8+ T cell subsets were activated with TNF-α for 10 min and NF-κB activity was measured by ELISA-based DNA binding activity. Data are shown from five young and five aged subjects. A significantly lower (P < 0.01) NF-κB activation was observed in aged subjects