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Fig. 1 | Immunity & Ageing

Fig. 1

From: NK cells of the oldest seniors represent constant and resistant to stimulation high expression of cellular protective proteins SIRT1 and HSP70

Fig. 1

Gating strategy for flow cytometric analyses of NK cells. a Forward scatter (FSC) and side scatter (SSC) characteristics of PBMC population with gated lymphocytes (G1). b NK cell gating – NK cells were defined as CD3 negative and CD56 positive population (G2). c NK cells expressing SIRT1 were identified in the upper right quadrant (Q2). d NK cells expressing SOD2 were identified in the upper right quadrant (Q2). e NK cells expressing intracellular HSP70 were identified in the upper right quadrant (Q2). f Isotype control for SIRT1 positive cells (Q3). g Isotype control for SOD2 positive cells (Q3). h Isotype control for HSP70 positive cells (Q3)

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Immunity & Ageing

ISSN: 1742-4933

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