Fig. 1

Effect of age on basophils in the bone marrow and the spleen. a Flow cytometric analysis of basophils in the BM, defined as live Lin⁻FcεRIα⁺CD117⁻CD200R3⁺. b Surface expression on BM basophils of CD200R3 and CD123. Representative example of a young (open blue) and an old mouse (open red). All BM cells from the same old (filled red) are shown in the CD200R3 plot. Isotype staining for CD123 is shown in grey. c Quantification of mean frequencies and absolute numbers of BM basophils or MFI on BM basophils of FcεRIα, CD200R3, IL-33R, and TSLPR. d Flow cytometric analysis of basophils in the spleen, defined as live Lin⁻FcεRIα⁺CD117⁻CD200R3⁺. e Surface expression on spleen basophils of CD200R3 and CD123. Representative example of a young (open blue) and an old mouse (open red). All spleen cells from the same old (filled red) are shown in the CD200R3 plot. Isotype staining for CD123 is shown in grey. f Quantification of mean frequencies and absolute numbers of spleen basophils or MFI on spleen basophils of FcεRIα, CD200R3, TSLPR, IL-33R, and CD11b. * = p < 0.05. Data represent n = 10 mice per group. BM = bone marrow; L/D = live/dead stain; Lin = lineage (CD3, CD4, CD8, CD11c, CD19, CD45R/B220, Ly6C/Ly6G (Gr-1), NK1.1, TER-119), with CD11b additionally in BM; MFI = median fluorescence intensity; SSC = side scatter