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Fig. 6 | Immunity & Ageing

Fig. 6

From: Aged mice display altered numbers and phenotype of basophils, and bone marrow-derived basophil activation, with a limited role for aging-associated microbiota

Fig. 6

Effect of age and age-related microbiota on pure bone marrow-derived basophils. a Representative example of purified BMB, defined as FcεRIα+CD11cCD117. b Flow cytometric analysis of intracellular Ki-67, IL-4, and IL-13 staining of CD200R3-stimulated BMB. For Ki-67, a representative example of a young (blue) and an old mouse (red) is given. c-d Effect of stimulation with different stimuli on expression of (extracellular) CD11b and (intracellular) Ki-67, IL-4, and IL-13 by BMB from young and aged mice (panel c) and by BMB from germ-free recipients from young and aged mice (panel d). Every stimulation condition, including the medium control, contained 2 ng/mL IL-3. * = p < 0.05; ** = p < 0.01. Asterisks above bars indicate outcome of Bonferroni post hoc tests. The outcome of TWA is indicated below the legend. Data represent 5 cultures per group (with each culture derived from a different mouse). BMB = bone marrow-derived basophils; O = microbiota derived from old mouse; TWA = two-way ANOVA; Y = microbiota derived from young mouse

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