Table 2 Used antibodies for flow cytometry and purification
Target | Format | Clone | Company |
|---|---|---|---|
CD3e | FITC | 145-2C11 | BD |
CD4 | FITC | H129.19 | BD |
CD8a | FITC | 53–6.7 | BD |
CD11b | BV421/FITC | M1/70 | BD |
CD11c | Biotin/FITC | HL3 | BD |
CD16/32 | FITC/Purified | 2.4G2 | BD |
CD19 | FITC | 1D3 | Ebioscience |
CD45R/B220 | FITC | RA3-6B2 | BD |
CD62L | APC-Cy7 | MEL-14 | BD |
CD117 | Biotin BV421 BV510 | 2B8 2B8 ACK2 | BD BioLegend BioLegend |
CD123 | Biotin PE | 5B11 5B11 | BD Ebioscience |
CD200R3 | APC | Ba13 | BioLegend |
FcεRIα | Biotin/PE-Cy7 | MAR-1 | Ebioscience |
IL-4 | APC | 11B11 | Ebioscience |
IL-13 | PE-Cy7 | eBio13A | Ebioscience |
IL-33R/ST2 | PerCP-eFluor710 | RMST2–2 | Ebioscience |
Ki-67 | FITC | SolA15 | Ebioscience |
Ly6C/Ly6G (Gr1) | FITC | RB6-8C5 | BD |
NK1.1 | FITC | PK136 | Ebioscience |
TER-119 | FITC | TER-119 | BD |
TSLPR | PE | R&D | |
Streptavidin | APC-eFluor780 | Ebioscience |