Fig. 2
From: Topological DNA damage, telomere attrition and T cell senescence during chronic viral infections
Top 1 inhibition induces telomeric DNA damage, T cell apoptosis, and T cell dysfunction. a Dose- and time-dependent induction of Av and 7-AAD expression in CD4 T cells treated with varying concentrations of CPT for 1, 2, and 3 days, analyzed by flow cytometry. b Inhibition of IL-2 and IFN-γ expression in CD4 T cells treated with or without 1 μM CPT for 3 days, followed by flow cytometry. c Flow cytometry of T cell proliferation as determined by PKH26 dye dilution in CD4 T cells treated with or without 1 μM CPT for 5 days. d γH2AX expression in CD4 T cells treated with or without 1 μM CPT for 3 days followed by flow cytometry. e Confocal microscopy of Telomeric DNA damage foci to assess co-localization of 53BP1 and TRF1 on telomeres, in CD4 T cells treated with or without 1 μM CPT for 2 days. Representative images and summary data for TIFs per nucleus as well as percentage of cells with > 3 TIFs are shown