Fig. 3
From: Topological DNA damage, telomere attrition and T cell senescence during chronic viral infections
Top 1 inhibition, Top1cc and PARP1 induction, and transcription-dependent ubiquitination of Top 1 in CPT-treated T cells. a Western blot analysis of Top 1 inhibition in CD4 T cells treated with or without 1 μM CPT for 3 days. b Top 1 enzyme activity measured in CD4 T cells exposed to CPT and control for 3 days. c Top1cc detected in genomic DNA from CD4 T cells treated with or without CPT (50 μM for 45 min). d PARP1 induction in CD4 T cells with or without CPT treatment. e Western blot analysis of Top 1, PARP1, and γH2AX levels in CD4 T cells with or without CPT treatment in the presence or absence of APRP-1 inhibitor (ABT-888). f Western blot analysis of Top 1, PARP1, and γH2AX levels in CD4 T cells with or without CPT treatment in the presence or absence of transcription inhibitor (FLV). g Western blot analysis of Top 1, PARP1, and γH2AX levels in CD4 T cells with or without CPT treatment in the presence or absence of ubiquitin isopeptidase inhibitor (G5). h Western blot analysis of Top 1, PARP1, and γH2AX levels in CD4 T cells with or without CPT treatment in the presence or absence of proteasomal inhibitor (MG-132)