Fig. 4
From: Topological DNA damage, telomere attrition and T cell senescence during chronic viral infections
Top 1 inhibition induces telomere erosion via suppression of telomerase activity and shelterin proteins. a Telomere length, measured by Flow-FISH, in CD4 T cells with or without CPT treatment for 5 days. b Telomerase hTERT expression, measured by real-time RT-PCR, in CD4 T cells treated with or without CPT. c Telomerase activity, measured by TRAP assay, in CD4 T cells treated with or without CPT. d Shelterin proteins, measured by western blot, in CD4 T cells treated with or without CPT. e Shelterin mRNA, measured by real-time RT-PCR, in CD4 T cells treated with or without CPT