Fig. 5
From: Topological DNA damage, telomere attrition and T cell senescence during chronic viral infections
Top 1-mediated DDR involves dynamic activation and deficiency of DNA repair kinases. a Western blot analysis of kinetic alterations in PARP1, γH2AX, pATM, ATM, CHK2, and ATR levels in CD4 T cells treated with or without CPT for different time points. Representative images and summary data from 3 independent experiments are shown. b Western blot of Top 1 and γH2AX expression in CD4 T cells treated with or without CPT in the presence or absence of ATMi or ATRi. c Representative images from confocal microscopy examination of co-localization of Ku70 and TRF1 in CD4 T cells treated with CPT or DMSO for 3 days. d Representative images of co-localization of RAD51 and TRF1 in CD4 T cells treated with CPT or DMSO for 3 days. e Representative images of co-localization of Top1cc and TRF1 in CD4 T cells treated with CPT or DMSO for 3 h