Table 2 PCR primers used in this study
From: Topological DNA damage, telomere attrition and T cell senescence during chronic viral infections
Primers | Forward | Reverse |
|---|---|---|
TOP1 | 5′-TCCGGAACCAGTATCGAGAAGA-3′ | 5′-CCTCCTTTTCATTGCCTGCTC-3’ |
hTERT | 5′- CCAAGTTCCTGCACTGGCTGA-3’ | 5′- TTCCCGATGCTGCCTGACC-3’ |
TRF1 | 5′-TGCTTTCAGTGGCTCTTCTG-3’ | 5′-ATGGAACCCAGCAACAAGAC-3’ |
TRF2 | 5′-GGTACGGGGACTTCAGACAG-3’ | 5′-CGCGACAGACACTGCATAAC-3’ |
TPP1 | 5′-TCACCAGATCAGCCACATTC-3’ | 5′-TGGAAAGACTCTCGGAGCTG-3’ |
POT1 | 5′-TTCCACTAAAGAGCAGGCAA-3’ | 5′-TGAAGTTCTTTAAGCCCCCA-3’ |
TIN2 | 5′-TGCTTTCAGTGGCTCTTCTG-3’ | 5′-TTTACCAGCAGGTGAAGCAG-3’ |
RAP1 | 5′-TCTTCTTCAGGCAAATCTGGA-3’ | 5′-CCTCCTCCCAGAAGCTCAA-3’ |
GAPDH | 5′-TGCACCACCAACTGCTTAGC-3’ | 5′- GGCATGGACTGTGGTCATGAG-3’ |