Fig. 2

AuNP-M2e + sCpG induces long term titers for all IgG subclasses. (a) Diagram containing all time points for this Fig. (b-f) M2e peptide was used as the coating antigen for ELISAs. Serum from specified mice and time point was added. M2e-specific titer was detected by an IgG subclass specific secondary antibody. Average background from naïve unvaccinated serum was subtracted out. OD 490 nm = Optical Density 490 nm. (b-e) n = 18–19 and (f) n = 11–12, One-way ANOVA with Tukey’s multiple comparison test. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001