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Fig. 4 | Immunity & Ageing

Fig. 4

From: AuNP-M2e + sCpG vaccination of juvenile mice generates lifelong protective immunity to influenza A virus infection

Fig. 4

AuNP-M2e + sCpG vaccination induces long lived M2e-specific B cells. (a) Diagram containing all time points for this Fig. (b-f) M2e peptide was used as the coating antigen for ELISAs. Serum from survivor mice from specified group and time point was added and M2e-specific titer was detected by an IgG subclass specific secondary antibody. Average background from naïve unvaccinated serum was subtracted out. OD 490 nm = Optical Density 490 nm. (b-e) n = 3 (Unvaccinated), 7 (2X) and 12 (3X) and (f) n = 3 (Unvaccinated), 2 (2X) and 9 (3X). (b-f) Paired T test. (g) Two groups of BALB/c mice (3.4 and 0.8 months at the time of the first vaccination) were vaccinated three times with AuNP-M2e + sCpG on the same days. Antibody titer tested by ELISA as described for B-F. n = 8–10 prior to infection for all time points, IgG subclasses, and groups, except 18.2 month old IgG3 (n = 2–3) and post-infection n varies between 1 and 8, Two-way ANOVA with Sidak’s multiple comparison test. * p < 0.05, ** p < 0.01

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