Fig. 2
Analysis of senescence in 14-day SF cultures. a SA-β-gal activity and DAPI staining. b Time-dependent expansion of SA-β-gal(+) in HSF cultures (n = 4) (*ANOVA). c Correlation between the frequency of SA-β-gal(+) SF and donor’s age (n = 11). d Frequency of SA-β-gal(+) from < 40 (n = 5) and > 40 (n = 6) HSF, and from > 40 OASF (n = 4) and > 40 RASF (n = 4) cultures (*Mann-Whitney). e CDKN1A and LMNB1 mRNA expression in < 40 (n = 3) and > 40 (n = 5) HSF cultures (*Mann-Whitney). f Frequency of SA-β-gal(+) from < 40 (n = 5) and > 40 (n = 6) HSF cultures treated with TNFα, H2O2 or untreated (CT) (*Friedman, ##Mann-Whitney)