Fig. 3

Aged donor HSCs exhibit reduced B lineage development in vitro. Sorted HSCs from young and aged donors (N = 8 each) were seeded at 100 cells/ well in triplicate cultures that support multilineage development and were assessed by flow cytometry after 3 weeks. a Total numbers of cells from young (open) and aged donor (closed) cultures are shown. Frequencies of CD33⁺ myeloid cells (b), CD19⁺ B lineage cells (c), and CD56⁺ NK cells (d) were evaluated. e A representative flow cytometry plot reveals co-expression of CD34 on some cells with lineage markers. f Total frequencies of CD34⁺ cells, as well as cells co-expressing mature lineage markers are shown. Means and standard error bars are presented. Statistical significance was determined by Mann Whittney U test; *, p = 0.038, **, p = 0.0019, ***, p < 0.0003