Fig. 5

Inhibition of ARID3a in young donor HSCs reduces B linage cell numbers. Young donor HSCs from Fig. 3 were untreated (media, circles), or transfected with lentivirus containing a scramble control shRNA (shCon, squares) or an ARID3a-specific shRNA (shA3a, triangles), and were cultured and assessed as in Fig. 4. a Relative transfection efficiencies at the end of the culture are indicated by GFP-co—expression. Total cell numbers (b) and frequencies of CD33⁺ myeloid cells (c), CD19⁺ B lineage cells (d), and CD56⁺ NK cells (e) are shown. Total frequencies of CD34⁺(f) and CD34-co-expressing lineage-directed cells are shown. Means and standard error bars are presented. Statistical significance was determined by Mann Whittney U test; *, p < 0.04, ***, p < 0.0003