Fig. 6

ARID3a⁺ HSCs from aged donors express altered transcriptomes compared to ARID3a⁺ HSCs from young donors. Single-cell RNA-seq expression profiles from 4 young (ages 19, 21, 37, and 40) and 4 aged (ages 61, 66, 68, and 70) donors were obtained and analyzed based on ARID3A transcript levels (ARID3A⁺ = > 0.5 CPM, ARID3A⁻ = < 0.5 CPM). Data from 264 young donor cells (172 ARID3A⁺ and 92 ARID3A⁻) and 301 aged donor cells (153 ARID3A⁺ and 148 ARID3A⁻) were assessed. a ARID3A transcript levels for young and aged donor cells are shown. tSNE plots of aged donor HSCs (circles) are overlaid with shaded young donor HSCs (squares) (b) and ARID3a expression levels are indicated by intensities of blue dots. In (c), tSNE plots are overlaid with young donor HSCs over the shaded aged donor HSCs for better visibility of the ARID3a-expressing cells in each group. The top GO terms enriched in DEGs from ARID3a⁺ vs ARID3a⁻ Aged HSCs are given with p values (d) and for young ARID3a⁺ versus ARID3a⁻ cells in (e). f Top GO terms directly comparing ARID3a⁺ cells in aged versus young donors are shown. g The most differentially expressed genes in ARID3a + HSCs in aged versus young donors are presented with negative or positive fold change (FC)