Fig. 2
From: Age-related changes in T lymphocytes of patients with head and neck squamous cell carcinoma
Expression levels of the ectonucleotidases CD39 and CD73 on CD4+ T cells in healthy donors and HNSCC patients in comparison as well as CD73 expression in response to T-cell stimulation. PBL were examined for surface expression of the ectonucleotidases CD39 and CD73 by FACS (a-b). The CD39 and CD73 characterization of CD4+ T cells of the healthy and tumor blood in representative density plots. The co-expression of CD39 and CD73 on CD4+ blood T cells were plotted for healthy donors and tumor patients and compared to CD4+ TIL (c). PBL were incubated with anti-CD3 and anti-CD28 for at least 2 days. The frequency of CD73 on CD8+, Treg and CD4+ T cells was measured by FACS before and after each day of stimulation (n = 4) (d). Data were analyzed with a Two-Way ANOVA and shown as mean; p < 0.05 (*); p < 0.01 (**); p < 0.001 (***)