Fig. 2
Splenic leukocyte composition is altered in a sex- and age-dependent manner. a Plots show frequency of B cells, monocytes, neutrophils, NK cells, CD4 + T cells, and CD8 + T cells in young and aged male and female mice. b Plots show total number of B cells, monocytes, neutrophils, NK cells, CD4 + T cells, and CD8 + T cell in young and aged male and female mice. Males are represented by blue circles and females are represented by pink squares. Young males (n = 6, 2–3 months); young females (n = 6, 2–3 months); aged males (n = 6, 21–22 months); aged females (n = 6, 21–22 months). Data were analyzed by 2-way ANOVA followed by Fisher’s LSD post hoc analysis. Data represent mean ± SEM. Comparisons within sex: *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Comparisons within age: #p < 0.05, ##p < 0.01, ###p < 0.001, ####p < 0.0001